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7000 thermal cycler  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 7000 thermal cycler
    7000 Thermal Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/7000+thermal+cycler/nct06102629-7-10-14
    Average 90 stars, based on 1 article reviews
    7000 thermal cycler - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The effect of prostate tissue inflammation in benign prostatic hyperplasia on enhancer of zeste homolog 2 ribonucleic acid expression
    Article Snippet: Standardized specific primers and the fluorescent taqman probe for the EZH2 gene were purchased from Applied Biosystems (Darmstadt, Germany). .. Real-time polymerase chain reaction (PCR) was carried using ABI 7000 thermal cycler (Applied Biosystems). ..

    Article Title: Understanding the Epigenetic Mechanisms in the Pathophysiology of Polycystic Ovary Syndrome for Efficient Diagnosis and Targeted Therapy
    Article Snippet: Measure Description: Total RNA from ovarian tissue will be extracted using TRIzol reagent according to the manufacturer's instructions (Invitrogen Life Technologies) and reverse transcribed in the presence of random hexamers. .. Quantitative real-time PCR reactions will be carried out in an ABI 7000 Thermal Cycler (Applied Biosystems) to analyse the expression of epigenetic markers including the androgen regulated miRNAs and their downstream targets.. Time Frame: 3 YEARS ..

    Article Title: The Relationship between Functional Promoter Variants of Macrophage Migration Inhibitory Factor and Endometriosis
    Article Snippet: .. Quantitative real-time PCR was performed in an ABI 7000 Thermal Cycler (Applied Biosystems, Foster City, CA, USA). .. Each standard PCR reaction contained 2 μl cDNA templates, 1 μl of each primer (final concentration, 0.1 mmol/L), and 10 μl SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) containing Taq DNA polymerase buffer, deoxynucleotide triphosphate mix, SYBR green I, MgCl2, and Taq DNA polymerase.

    Polymerase Chain Reaction:

    Article Title: The effect of prostate tissue inflammation in benign prostatic hyperplasia on enhancer of zeste homolog 2 ribonucleic acid expression
    Article Snippet: Standardized specific primers and the fluorescent taqman probe for the EZH2 gene were purchased from Applied Biosystems (Darmstadt, Germany). .. Real-time polymerase chain reaction (PCR) was carried using ABI 7000 thermal cycler (Applied Biosystems). ..

    Article Title: The Indoleamine-2,3-Dioxygenase (IDO) Inhibitor 1-Methyl-D-tryptophan Upregulates IDO1 in Human Cancer Cells
    Article Snippet: Total RNA was isolated with the Qiagen RNAeasy RNA isolation kit (Hilden, Germany) and DNA was synthesized with the Applied Biosystems reverse-transcription-Kit (Foster City, CA, USA) according to manufacturer's instructions. .. QRT-PCR was preformed in an ABI 7000 thermal cycler with SYBR Green PCR Mastermix (Applied Biosystems) according to standard protocols. ..

    Expressing:

    Article Title: Understanding the Epigenetic Mechanisms in the Pathophysiology of Polycystic Ovary Syndrome for Efficient Diagnosis and Targeted Therapy
    Article Snippet: Measure Description: Total RNA from ovarian tissue will be extracted using TRIzol reagent according to the manufacturer's instructions (Invitrogen Life Technologies) and reverse transcribed in the presence of random hexamers. .. Quantitative real-time PCR reactions will be carried out in an ABI 7000 Thermal Cycler (Applied Biosystems) to analyse the expression of epigenetic markers including the androgen regulated miRNAs and their downstream targets.. Time Frame: 3 YEARS ..

    Reverse Transcription:

    Article Title: LncRNA SPRY4‐IT1 facilitates cell proliferation and angiogenesis of glioma via the miR‐101‐3p/EZH2/VEGFA signaling axis
    Article Snippet: Total RNA was isolated from the cells and tissues by a TRIZOL kit (Invitrogen) based on the manufacturer's protocol. .. RNA was quantified and then reverse‐transcribed into cDNA by a cDNA synthesis kit (Novoprotein). qRT‐PCR was conducted by SYBR Green (Novoprotein) in an ABI 7000 thermal cycler. ..

    cDNA Synthesis:

    Article Title: LncRNA SPRY4‐IT1 facilitates cell proliferation and angiogenesis of glioma via the miR‐101‐3p/EZH2/VEGFA signaling axis
    Article Snippet: Total RNA was isolated from the cells and tissues by a TRIZOL kit (Invitrogen) based on the manufacturer's protocol. .. RNA was quantified and then reverse‐transcribed into cDNA by a cDNA synthesis kit (Novoprotein). qRT‐PCR was conducted by SYBR Green (Novoprotein) in an ABI 7000 thermal cycler. ..

    SYBR Green Assay:

    Article Title: LncRNA SPRY4‐IT1 facilitates cell proliferation and angiogenesis of glioma via the miR‐101‐3p/EZH2/VEGFA signaling axis
    Article Snippet: Total RNA was isolated from the cells and tissues by a TRIZOL kit (Invitrogen) based on the manufacturer's protocol. .. RNA was quantified and then reverse‐transcribed into cDNA by a cDNA synthesis kit (Novoprotein). qRT‐PCR was conducted by SYBR Green (Novoprotein) in an ABI 7000 thermal cycler. ..

    Article Title: The Indoleamine-2,3-Dioxygenase (IDO) Inhibitor 1-Methyl-D-tryptophan Upregulates IDO1 in Human Cancer Cells
    Article Snippet: Total RNA was isolated with the Qiagen RNAeasy RNA isolation kit (Hilden, Germany) and DNA was synthesized with the Applied Biosystems reverse-transcription-Kit (Foster City, CA, USA) according to manufacturer's instructions. .. QRT-PCR was preformed in an ABI 7000 thermal cycler with SYBR Green PCR Mastermix (Applied Biosystems) according to standard protocols. ..

    Quantitative RT-PCR:

    Article Title: Macrophage Migration Inhibitory Factor Antagonist Blocks the Development of Endometriosis In Vivo
    Article Snippet: .. Using the standard Trizol® protocol (Invitrogen) as previously reported , RNA was extracted separately from each surface and deep implant. qRT-PCR was performed using an ABI 7000 Thermal Cycler (Applied Biosystems, Foster City, CA). .. Each standard PCR reaction contained 2 μl reverse transcriptase (RT) product, 0.5 μl of each primer (final concentration, 0.1 m m ), 12.5 μl SYBR Green PCR Master Mix (Invitrogen) consisting of Taq DNA polymerase reaction buffer, deoxynucleotide triphosphate mix, SYBR green I, MgCl 2 , and Taq DNA polymerase.

    Article Title: Abnormal Expression of Prostaglandins E2 and F2 α Receptors and Transporters in Patients with Endometriosis
    Article Snippet: Total RNA was extracted from endometrial tissue using the TRIzol reagent according to the manufacturer's instructions (Invitrogen Life Technologies, Inc., Grand Island, NY, USA) and reverse-transcribed in the presence of random hexamers. .. The qRT-PCR reaction was carried out in an ABI 7000 Thermal Cycler (Applied Biosystems, Foster City, CA, USA). .. The standard reaction mixture contained 2 μ L of RT product, 0.5 μ L of each primer (final concentration, 0.1 mM), 12.5 μ L SYBR Green PCR Master Mix (Invitrogen Life technologies, Inc., Grand Island, NY, USA) consisting of Taq DNA polymerase reaction buffer, dNTP mix, SYBR green I, MgCl 2 , and Taq DNA polymerase.



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